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human embryonic lung fibroblast cell line mrc 5  (ATCC)


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    Structured Review

    ATCC human embryonic lung fibroblast cell line mrc 5
    Human Embryonic Lung Fibroblast Cell Line Mrc 5, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 5423 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/embryonic+lung+fibroblast+cell+line+mrc+5/MRC-5/pm42271325-65-0-8
    Average 99 stars, based on 5423 article reviews
    human embryonic lung fibroblast cell line mrc 5 - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Derivative Assay:

    Article Title: Mitogen-activated protein kinases-dependent induction of hepatocyte growth factor production in human dermal fibroblasts by the antibiotic polymyxin B.
    Article Snippet: Hepatocyte growth factor (HGF) stimulates migration and proliferation of keratinocytes and has been suggested to be involved in wound healing.. The cationic antibiotic polymyxin B (PMB) is commonly used as a topical antibiotic for wound care.. If PMB possesses an HGF-inducing activity, the antibiotic is potentially beneficial for wound healing in addition to minimizing chances of infection.

    Cell Culture:

    Article Title: Mitogen-activated protein kinases-dependent induction of hepatocyte growth factor production in human dermal fibroblasts by the antibiotic polymyxin B.
    Article Snippet: Hepatocyte growth factor (HGF) stimulates migration and proliferation of keratinocytes and has been suggested to be involved in wound healing.. The cationic antibiotic polymyxin B (PMB) is commonly used as a topical antibiotic for wound care.. If PMB possesses an HGF-inducing activity, the antibiotic is potentially beneficial for wound healing in addition to minimizing chances of infection.

    Article Title: LAPTM4B Alleviates Pulmonary Fibrosis by Enhancing NEDD4L-Mediated TGF-β Signaling Suppression
    Article Snippet: Human non-small cell lung cancer cell line A549 was obtained from Procell (Wuhan, China) and cultured in DMEM/F12 medium (PM150310, Procell, Wuhan China), supplemented with 10% FBS (Gibco, Grand Island, NY), 100 U/mL penicillin, and 100 μg/mL streptomycin (Solarbio, Beijing, China). .. The human embryonic lung fibroblast cell line MRC-5, obtained from ATCC, was cultured in MEM (Hyclone) supplemented with 10% FBS, 2 mM L-glutamine, 100 U/mL penicillin, and 100 μg/mL streptomycin (Solarbio, Beijing, China). .. HEK293T cells, also obtained from Procell (Wuhan, China), were cultured in high-glucose DMEM (Hyclone) supplemented with 10% FBS, 2 mM L-glutamine, 100 U/mL penicillin, and 100 μg/mL streptomycin.

    Article Title: Aging-Associated Metabolite Methylmalonic Acid Increases Susceptibility to Pulmonary Fibrosis.
    Article Snippet: opyright a 2024 American Society for Inves echnologies. ttps://doi.org/10.1016/j.ajpath.2024.04.011 Idiopathic pulmonary fibrosis (IPF) is a progressive interstitial lung disease characterized by pulmonary fibroblast overactivation, resulting in the accumulation of abnormal extracellular matrix and lung parenchymal damage.. Although the pathogenesis of IPF remains unclear, aging was proposed as the most prominent nongenetic risk factor.. Propionate metabolism undergoes reprogramming in the aging population, leading to the accumulation of the by-product methylmalonic acid (MMA).

    Mutagenesis:

    Article Title: HIF-1α siRNA Enhances the Efficacy of Erlotinib in Non-small Cell Lung Cancer: A Novel Strategy to Reverse Hypoxia-Induced Drug Resistance
    Article Snippet: .. The human embryonic lung fibroblast cell line MRC-5 (RRID: CVCL_0440) and the non-small cell lung cancer cell lines A549 (KRAS mutation, EGFR wild-type, RRID: CVCL_0023) and H1975 (L858R and T790M mutations, RRID: CVCL_1511) were purchased from the Shanghai Cell Bank of the Chinese Academy of Sciences (China), originally provided by the American Type Culture Collection, where these lines were authenticated using morphology, karyotyping, and polymerase chain reaction (PCR)-based approaches and tested for mycoplasma. ..

    Article Title: HIF-1α siRNA Enhances the Efficacy of Erlotinib in Non-small Cell Lung Cancer: A Novel Strategy to Reverse Hypoxia-Induced Drug Resistance.
    Article Snippet: Item Company City, country Catalog no. Erlotinib Titan Scientific Co., Ltd. Shanghai, China 041178861 Small interfering RNA TsingKe Biotech Co., Ltd. Beijing, China NA siRNA-mate Plus Transfection Kit GenePharma Co., Ltd. Suzhou, China G04026 ABScript III RT Master Mix for qPCR with gDNA Remover Aibotek Biotechnology Co., Ltd. Wuhan, China RK20429 ABScript II One Step SYBR Green RT-qPCR Kit Aibotek Biotechnology Co., Ltd. Wuhan, China RK20404 RIPA lysis buffer Beyotime Biotech Inc. Shanghai, China P0013B BCA protein assay kit Beyotime Biotech Inc. Shanghai, China P0012 SDS-PAGE gel preparation kit Beyotime Biotech Inc. Shanghai, China P0012A PVDF membranes Beyotime Biotech Inc. Shanghai, China FFP71 L-lactate assay kit Beyotime Biotech Inc. Shanghai, China S0208S HIF-1α antibody Cell Signaling Technology, Inc. Danvers, MA, USA 3716S β-Actin antibody Cell Signaling Technology, Inc. Danvers, MA, USA 4967S Item Company City, country Catalog no. β-Actin rabbit mAb (HRP conjugate) Cell Signaling Technology, Inc. Danvers, MA, USA 5125S Cell Counting Kit-8 Yeasen Biotechnology Co., Ltd. Shanghai, China 40203ES80 Transwell Chambers Corning Inc. Corning, NY, USA 3401 DAPI staining reagent Merck KGaA Darmstadt, Germany 28718-90-3 HIF-1α hypoxia-inducible factor-1α, HRP horseradish peroxidase, mAb monoclonal antibody, NA not applicable, qPCR quantitative polymerase chain reaction .. The human embryonic lung fibroblast cell line MRC-5 (RRID: CVCL_0440) and the non-small cell lung cancer cell lines A549 (KRAS mutation, EGFR wild-type, RRID: CVCL_0023) and H1975 (L858R and T790M mutations, RRID: CVCL_1511) were purchased from the Shanghai Cell Bank of the Chinese Academy of Sciences (China), originally provided by the American Type Culture Collection, where these lines were authenticated using morphology, karyotyping, and polymerase chain reaction (PCR)-based approaches and tested for mycoplasma. ..

    Polymerase Chain Reaction:

    Article Title: HIF-1α siRNA Enhances the Efficacy of Erlotinib in Non-small Cell Lung Cancer: A Novel Strategy to Reverse Hypoxia-Induced Drug Resistance
    Article Snippet: .. The human embryonic lung fibroblast cell line MRC-5 (RRID: CVCL_0440) and the non-small cell lung cancer cell lines A549 (KRAS mutation, EGFR wild-type, RRID: CVCL_0023) and H1975 (L858R and T790M mutations, RRID: CVCL_1511) were purchased from the Shanghai Cell Bank of the Chinese Academy of Sciences (China), originally provided by the American Type Culture Collection, where these lines were authenticated using morphology, karyotyping, and polymerase chain reaction (PCR)-based approaches and tested for mycoplasma. ..

    Article Title: HIF-1α siRNA Enhances the Efficacy of Erlotinib in Non-small Cell Lung Cancer: A Novel Strategy to Reverse Hypoxia-Induced Drug Resistance.
    Article Snippet: Item Company City, country Catalog no. Erlotinib Titan Scientific Co., Ltd. Shanghai, China 041178861 Small interfering RNA TsingKe Biotech Co., Ltd. Beijing, China NA siRNA-mate Plus Transfection Kit GenePharma Co., Ltd. Suzhou, China G04026 ABScript III RT Master Mix for qPCR with gDNA Remover Aibotek Biotechnology Co., Ltd. Wuhan, China RK20429 ABScript II One Step SYBR Green RT-qPCR Kit Aibotek Biotechnology Co., Ltd. Wuhan, China RK20404 RIPA lysis buffer Beyotime Biotech Inc. Shanghai, China P0013B BCA protein assay kit Beyotime Biotech Inc. Shanghai, China P0012 SDS-PAGE gel preparation kit Beyotime Biotech Inc. Shanghai, China P0012A PVDF membranes Beyotime Biotech Inc. Shanghai, China FFP71 L-lactate assay kit Beyotime Biotech Inc. Shanghai, China S0208S HIF-1α antibody Cell Signaling Technology, Inc. Danvers, MA, USA 3716S β-Actin antibody Cell Signaling Technology, Inc. Danvers, MA, USA 4967S Item Company City, country Catalog no. β-Actin rabbit mAb (HRP conjugate) Cell Signaling Technology, Inc. Danvers, MA, USA 5125S Cell Counting Kit-8 Yeasen Biotechnology Co., Ltd. Shanghai, China 40203ES80 Transwell Chambers Corning Inc. Corning, NY, USA 3401 DAPI staining reagent Merck KGaA Darmstadt, Germany 28718-90-3 HIF-1α hypoxia-inducible factor-1α, HRP horseradish peroxidase, mAb monoclonal antibody, NA not applicable, qPCR quantitative polymerase chain reaction .. The human embryonic lung fibroblast cell line MRC-5 (RRID: CVCL_0440) and the non-small cell lung cancer cell lines A549 (KRAS mutation, EGFR wild-type, RRID: CVCL_0023) and H1975 (L858R and T790M mutations, RRID: CVCL_1511) were purchased from the Shanghai Cell Bank of the Chinese Academy of Sciences (China), originally provided by the American Type Culture Collection, where these lines were authenticated using morphology, karyotyping, and polymerase chain reaction (PCR)-based approaches and tested for mycoplasma. ..

    Modification:

    Article Title: Aging-Associated Metabolite Methylmalonic Acid Increases Susceptibility to Pulmonary Fibrosis.
    Article Snippet: opyright a 2024 American Society for Inves echnologies. ttps://doi.org/10.1016/j.ajpath.2024.04.011 Idiopathic pulmonary fibrosis (IPF) is a progressive interstitial lung disease characterized by pulmonary fibroblast overactivation, resulting in the accumulation of abnormal extracellular matrix and lung parenchymal damage.. Although the pathogenesis of IPF remains unclear, aging was proposed as the most prominent nongenetic risk factor.. Propionate metabolism undergoes reprogramming in the aging population, leading to the accumulation of the by-product methylmalonic acid (MMA).



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